Codon degeneracy contributes to divergent fitness effects of rare tRNAs with A-starting anticodons
This study demonstrates that engineered tRNAs with unmodified adenine at the wobble position are translationally active but exhibit divergent fitness effects in *E. coli*, where they are neutral or beneficial in four-fold degenerate codon boxes due to superwobbling, yet deleterious in two-fold degenerate boxes likely due to amino acid misincorporation.